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KMID : 0861020130280010009
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Korea Journal of Herbology
2013 Volume.28 No. 1 p.9 ~ p.14
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Effects of Bletillae Rhizoma on the Elastase, Collagenase, and Tyrosinase Activities and the Procollagen Synthesis in Hs68 Human Fibroblasts
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Lee Jung-Hun
Kim Myung-Gyou
Lee Se-Na
Leem Kang-Hyun
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Abstract
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Objectives： Bletillae Rhizoma, the roots of Bletilla striata, is used to restrain the leakage of blood and stop
bleeding. It can cure the sores, ulcers, and chapped skin. This study was designed to investigate the collagen
metabolism, elastase and tyrosinase activity of Bletillae Rhizoma extract (BR).
Methods : The effects of BR on type I procollagen production and collagenase activity in human normal
fibroblasts Hs68 after UVB (312 nm) irradiation were measured by ELISA method. The elastase activity,
tyrosinase activity, and L?DOPA oxidation after treatment of BR were measured as well.
Results : In the present study, the collagen production (type I procollagen) was significantly increased to 15.7
± 1.8 ng/ml at a concentration of BR 100 μg/ml in UVB damaged Hs68 cells. The increased collagenase
activity after UVB damage was significantly recovered to 42.7 ± 0.7%, 54.5 ± 3.5%, and 38.4 ± 0.9% by BR
10, 30, and 100 μg/ml. The activities of BR 10 mg/ml on tyrosinase activity was significantly reduced to 45.1
± 8.4% as well. However, there were no significant effects on the elastase activity and the L?DOPA oxidation
Conclusion : BR showed the promoting effects of collagen synthesis and inhibitory effects of collagenase activity
in Hs68, human normal fibroblast cells. And these could be thought to have the anti?wrinkle effects and
whitening effects in vitro. These results suggest that BR may have potential as an anti?aging ingredient in
cosmetic treatment.
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KEYWORD
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Bletillae Rhizoma, Elastase, Collagenase, Tyrosinase, Collagen, Hs68, Fibroblasts
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